体能科学

跑台运动对慢性应激大鼠学习记忆及海马CRH水平和多巴胺信号通路的影响

申贺元 (中北大学体育学院), 王鑫军 (中北大学体育学院), 王奕坤 (中北大学体育学院), 刘义豪 (中北大学体育学院), 郭燕兰 (中北大学体育学院)

摘要


目的：探讨4周跑台运动对慢性不可预知性温和应激（CUMS）大鼠空间学习记忆能力、海马促肾上腺皮质激素释放激素（CRH）及多巴胺（DA）水平和多巴胺受体表达的影响。方法：将36只大鼠随机分为3组：即对照组、应激模型组及应激运动组，每组12只。应激模型组及应激运动组大鼠每日进行CUMS 1次，连续28d，同时应激运动组大鼠进行4周跑台运动。跑台运动结束后用水迷宫实验评估大鼠空间记忆能力，应用ELISA法检测海马组织CRH水平，高效液相色谱-电化学法（HPLC-EC）检测海马组织DA水平，利用Westernblot法检测大鼠海马区多巴胺D1、D2受体的表达。结果：（1）与对照组比较，水迷宫实验中应激模型组大鼠逃避潜伏期及到达平台的游泳距离均明显延长，目标象限时间百分比及穿越站台次数均明显减少；海马组织CRH水平增加，DA水平及多巴胺D1受体（D1R）及D2受体（D2R）蛋白表达均明显减少（P均＜0.01）。（2）与应激模型组比较，水迷宫实验中应激运动组大鼠水迷宫训练3～5d逃避潜伏期及到达平台的游泳距离均显著缩短，目标象限的时间百分比及穿越站台次数明显增多；海马组织CRH水平下降，DA水平、D1R及D2R蛋白表达水平明显增加（P＜0.05，P＜0.01）。结论：长期跑台运动可改善CUMS大鼠的学习记忆能力，可能与此运动减少海马CRH释放、增加海马DA水平及激活D1R及D2R蛋白表达有关。

关键词


跑台运动；慢性不可预知温和应激；学习记忆；海马；促肾上腺皮质激素释放激素；多巴胺；多巴胺受体

全文:

PDF

参考


傅程龙,徐迅,郭燕兰,等.跑台运动对慢性应激大鼠海马sigma-1受体和内质网应激通路的影响[J].神经解剖学杂志,2025,41(1):39-44.

Bahramzadeh Zoeram S,Elahdadi Salmani M,Lashkarbolouki T,et al.Hippocampal orexin receptor blocking prevented the stress induced social learning and memory deficits[J].Neurobiol Learn Mem,2019(157):12-23.

Szczepanik JC,de Almeida GRL,Cunha MP,et al.Repeated methylglyoxal treatment depletes dopamine in the prefrontal cortex, and causes memory impairment and depressive-like behavior in mice[J].Neurochem Res,2020,45(2):354-370.

Amiri S,Amini-Khoei H,Mohammadi-Asl A,et al.Involvement of D1 and D2 dopamine receptors in the antidepressant-like effects of selegiline in maternal separation model of mouse[J].Physiol Behav,2016(163):107-114.

谢晶晶,黄锟.应激、海马促肾上腺皮质激素释放激素与学习记忆[J].卫生研究,2020,49(2):327-330.

Hupalo S,Bryce CA,Bangasser DA,et al.Corticotropin-Releasing Factor (CRF) circuit modulation of cognition and motivation[J].Neurosci Biobehav Rev,2019(103):50-59.

崔建梅,杨文艳,药宏慧,等.有氧运动对慢性应激大鼠空间学习记忆及海马齿状回BDNF表达的影响[J].首都体育学院学报,2018,30(1):78-82.

黄小莹,张黎君.高效液相色谱-电化学法测定大鼠脑脊液中多巴胺含量的研究[J].企业技术开发,2016,35(12):68-69.

Chen Y,Bender RA,Brunson KL,et al.Modulation of dendritic differentiation by corticotropin-releasing factor in the developing hippocampus[J].Proc Natl Acad Sci U S A,2004,101(44):15782-7.

Henckens MJ,Deussing JM,Chen A.Region-specific roles of the corticotropin-releasing factor-urocortin system in stress[J].Nat Rev Neurosci,2016,17(10):636-51.

唐彦,徐寅,张值伟,等.龙牡桂枝汤对慢性应激状态下ADHD大鼠学习记忆及海马-HPA轴的影响[J].中华中医药杂志,2024,39(1):382-386.

Othman MZ,Hassan Z,Che Has AT.Morris water maze: a versatile and pertinent tool for assessing spatial learning and memory[J].Exp Anim,2022,71(3):264-280.

Oran DS,Eraslan E.The effects of stress and environmental enrichment on cognitive functions and stress-related gene expressions in the brain of aged rats[J].Gen Physiol Biophys,2025,44(2):151-162.

Heissel A,Heinen D,Brokmeier LL,et al.Exercise as medicine for depressive symptoms? A systematic review and meta-analysis with meta-regression[J].Br J Sports Med,2023,57(16):1049-1057.

Mahdirejei HA,Peeri M,Azarbayjani MA,et al.Fluoxetine combined with swimming exercise synergistically reduces lipopolysaccharide-induced depressive-like behavior by normalizing the HPA axis and brain inflammation in mice[J].Pharmacol Biochem Behav,2023(232):173640.

Sheline YI,Liston C,McEwen BS.Parsing the hippocampus in depression: chronic stress, hippocampal volume, and major depressive disorder[J].Biol Psychiatry,2019,85(6):436-438.

March JS,Silva S,Petrycki S,et al.The Treatment for adolescents with depression study (TADS): long-term effectiveness and safety outcomes[J].Arch Gen Psychiatry,2007,64(10):1132-43.

Bortolotto VC,Pinheiro FC,Araujo SM,et al.Chrysin reverses the depressive-like behavior induced by hypothyroidism in female mice by regulating hippocampal serotonin and dopamine[J].Eur J Pharmacol,2018(822):78-84.

Yuan TT,Qiao H,Dong SP,et al.Activation of hippocampal D1 dopamine receptor inhibits glutamate-mediated depression induced by chronic unpredictable mild stress in rats[J].Sheng Li Xue Bao,2011,63(4):333-341.

Karailiev P,Hlavacova N,Chomanic P,et al.Dopamine concentrations and dopamine receptor gene expression in emotion-related brain structures of female adult rats exposed to stress of chronic isolation from weaning[J].Gen Physiol Biophys,2020,39(4):393-398.

Wang YC,Ho UC,Ko MC,et al.Differential neuronal changes in medial prefrontal cortex, basolateral amygdala and nucleus accumbens after postweaning social isolation[J].Brain Struct Funct,2012,217(2):337-51.

Izzo E,Sanna PP,Koob GF.Impairment of dopaminergic system function after chronic treatment with corticotropin-releasing factor[J].Pharmacol Biochem Behav,2005,81(4):701-8.

Payer D,Williams B,Mansouri E,et al.Corticotropin-releasing hormone and dopamine release in healthy individuals[J].Psychoneuroendocrinology,2017(76):192-196.

谢晶晶,黄锟.应激、海马促肾上腺皮质激素释放激素与学习记忆[J].卫生研究,2020,49(2):327-330.




DOI: https://doi.org/10.33142/jscs.v6i1.18538

Refbacks

  • 当前没有refback。


版权所有（c）{$ COPYRIGHTYEAR} {$ copyrightHolder}

Creative Commons License
此作品已接受知识共享署名-非商业性使用 4.0国际许可协议的许可。